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["Proprietary and Business Sensitive Document"]
["DE-FOA-0002423: Feedstocks and Algae Research Technologies \nTopic Area 2: APEX"]
["Instructions: Please Read","List the Task number as stated in the proposal.","List the title for each proposed task as it is described in the written proposal document."," List 1-2 performance metrics or parameters (key performance parameters (KPPs)) per task in separate rows that will be used to measure success of the proposed task.  ","List the current state of technology or KPP measurement that has been achieved or demonstrated to-date. Use this column to establish the baseline value.","List an intermediate target that builds upon the Baseline Measurement and can be used to assess progress of the proposed approach.","List the proposed end target of the approach. This should be the goal of the proposed task over the funding period and uses the same parameter as the Baseline Measurement and Intermediate Target listed.","List the proposed stretch target of the approach. This should be the goal of the proposed task over the funding period and uses the same parameter as the Baseline Measurement and Intermediate Target listed. It should be measured in an outdoor environment, not simulated conditions.","List the units of the measured parameter, if applicable.","Describe how the KPP Baseline Measurement was determined. For unit operation tests/experiments include hours of steady-state operation and important operating conditions.","List the duration and scale of the Baseline, Intermediate, and Target KPP (e.g., Bench/Lab/Flasks, ePBR, pond volume in liters, pilot or integrated pilot)"]
[null,"Task Number","Title of Proposed Task","Key Performance Parameter (KPP)","Baseline Measurement","Intermediate Target ","Proposal End Target","Project Stretch Target (APEX Outdoor Cultivation Challenge)","Units ","Data Basis / Measurement Description","Baseline Measurement Duration and Scale","Intermediate Target Duration and Scale","Proposed End Target Duration and Scale","Proposed Stretch Target (APEX Outdoor Cultivation Challenge) Duration and Scale"]
["The orange rows must be filled out per SOPO/project targets. The text provided is for example ONLY and must be replaced with project-specific KPPs per task.",1,"Improve productivity of Fantastic strain","Biomass productivity",18,20,22,22,"Ash-free dry weight of algae produced per square meter per day (g/m2/d)","(1) Biomass productivity test protocols will be requested during the  validation process and should include details on climate parameters (i.e.,. temperature and light) used in growth studies.\n\n(2) Test protocols will be requested and should include the \"data basis / measurement description\" for biomass productivity values. \n\n(3) Outdoor Productivities should be calculated using the 2016 SOT  \"Experimental duration – grow out – final harvest\" calculation protocol outlined in the 2016 SOT report  (Knoshaug, et al., Use of Cultivation Data ... State of Technology Assessments, NREL, October 2016). Outdoor cultivation trials should be a minimum of 2 weeks in duration.","Duration: 28 days\n\nScale:  500 mL ePBRs ","Duration: 28 days\n\nScale:  500 mL ePBRs ","Duration: 28 days\n\nScale:  500 mL ePBRs ","Duration: 28 days\n\nScale:  4.2 m2  outdoor ponds"]
[null,1,"Improve productivity of Fantastic strain","Biomass composition (quality)","Algae Strain: Strain A\nAsh (wt.%): 20\nProtein (wt.%): 42\nFAME lipid (wt.%): 15\nTotal Carbohydrates (wt.%): 18\n","Algae Strain: Strain A\nAsh (wt.%): 0\nProtein (wt.%): 42\nFAME lipid (wt.%): 35\nTotal Carbohydrates (wt.%): 18\n","Algae Strain: Strain A\nAsh (wt.%): 0\nProtein (wt.%): 42\nFAME lipid (wt.%): 35\nTotal Carbohydrates (wt.%): 18\n","Algae Strain: Strain A\nAsh (wt.%): 0\nProtein (wt.%): 42\nFAME lipid (wt.%): 35\nTotal Carbohydrates (wt.%): 18\n","wt.%","The biomass composition \"data basis / measurement description\" should be the same as the productivity data.","Duration: 28 days\n\nScale:  500 mL ePBRs ","Duration: 28 days\n\nScale:  500 mL ePBRs ","Duration: 28 days\n\nScale:  500 mL ePBRs ","Duration: 28 days\n\nScale:  4.2 m2  outdoor ponds"]
[null,1,"Improve productivity of Fantastic strain","SUPER gene and protein expression","No SUPER gene expression","mRNA expression of SUPER gene","Expressed SUPER protein and expected phenotype","Expressed SUPER protein and expected phenotype cultivated outdoors","N/A","PCR","lab-scale/flask","lab-scale/flask","lab-scale/flask and 500 mL ePBRs","lab-scale/flask and 500 mL ePBRs"]
[null,2,"Improving Amazing strain through designer microbial ecology","Biomass productivity","\"Axenic\" culture: baseline productivity = 18","Microbial consortium with algae: productivity = 20","Microbial consortium with algae: productivity = 22","Outdoor microbial consortium with algae: productivity = 22","Ash-free dry weight of algae produced per square meter per day (g/m2/d)","(1) Biomass productivity test protocols will be requested during the  validation process and should include details on climate parameters (i.e.,. temperature and light) used in growth studies.\n\n(2) Test protocols will be requested and should include the \"data basis / measurement description\" for biomass productivity values. \n\n(3) Outdoor Productivities should be calculated using the 2016 SOT  \"Experimental duration – grow out – final harvest\" calculation protocol outlined in the 2016 SOT report  (Knoshaug, et al., Use of Cultivation Data ... State of Technology Assessments, NREL, October 2016).","Duration: 28 days\n\nScale:  500 mL ePBRs ","Duration: 28 days\n\nScale:  500 mL ePBRs ","Duration: 28 days\n\nScale:  500 mL ePBRs ","Duration: 28 days\n\nScale:  4.2 m2  outdoor ponds"]
[null,2,"Improving Amazing strain through designer microbial ecology","Biomass composition ","Algae Strain: Amazing grown alone\nAsh (wt.%): 20\nProtein (wt.%): 42\nFAME lipid (wt.%): 15\nTotal Carbohydrates (wt.%): 18\n","Algae Strain: Amazing grown with consortium\nAsh (wt.%): 20\nProtein (wt.%): 42\nFAME lipid (wt.%): 15\nTotal Carbohydrates (wt.%): 18\n","Algae Strain: Amazing grown with consortium\nAsh (wt.%): 20\nProtein (wt.%): 42\nFAME lipid (wt.%): 15\nTotal Carbohydrates (wt.%): 18\n","Algae Strain: Amazing grown with consortium\nAsh (wt.%): 20\nProtein (wt.%): 42\nFAME lipid (wt.%): 15\nTotal Carbohydrates (wt.%): 18\n","wt.%","The biomass composition \"data basis / measurement description\" should be the same as the productivity data.","Duration: 28 days\n\nScale:  500 mL ePBRs ","Duration: 28 days\n\nScale:  500 mL ePBRs ","Duration: 28 days\n\nScale:  500 mL ePBRs ","Duration: 28 days\n\nScale:  4.2 m2  outdoor ponds"]
[null,3,"Gene annotation toolkit development","Accurate biological pathway mapping","No pathway maps for Fantastic strain"," Identification of the potential genes/proteins involved in biological pathway affected by SUPER gene expression","Full biological pathway map of pathways affected by SUPER gene expression","Full biological pathway map of pathways affected by SUPER gene expression","N/A","Mapping visualization; sequence alignments","Mapping software; computational analysis","Mapping software; computational analysis","Mapping software; computational analysis","Mapping software; computational analysis"]
["Add rows and Insert additional project specific KPPs, if required"]
["In addition to the orange rows, please fill in the green rows with the FOA Specific Key Performance Metrics, as listed. Fill out every row, and do not add or delete rows.","FOA",null,"Biomass productivity",{"$nulls":4},"Ash-free dry weight of algae produced per square meter per day (g/m2/d)",null,"Duration: \n\nScale: ","Duration: \n\nScale:  ","Duration: \n\nScale:  ","Duration: \n\nScale:  "]
[null,"FOA",null,"Biomass composition ","Algae Strain:\nAsh (wt.%):\nProtein (wt.%):\nLipid (wt.%):\nTotal Carbohydrates (wt.%):\n","Algae Strain:\nAsh (wt.%):\nProtein (wt.%):\nLipid (wt.%):\nTotal Carbohydrates (wt.%):\n","Algae Strain:\nAsh (wt.%):\nProtein (wt.%):\nLipid (wt.%):\nTotal Carbohydrates (wt.%):\n","Algae Strain:\nAsh (wt.%):\nProtein (wt.%):\nLipid (wt.%):\nTotal Carbohydrates (wt.%):\n","wt.%","The biomass composition \"data basis / measurement description\" should be the same as the productivity data.","Duration: \n\nScale: ","Duration: \n\nScale:  ","Duration: \n\nScale:  ","Duration: \n\nScale:  "]
[null,"FOA",null,"Calculated fuel yield based on achieved productivity and biomass composition",{"$nulls":4},"gasoline-gallon equivalency (GGE)",null,"NA","NA","NA","NA"]
[null,"FOA",null,"Media composition","Nitrogen source:\n[N]:\n[P]:\npH:\nOther nutrients/trace elements:","Nitrogen source:\n[N]:\n[P]:\npH:\nOther nutrients/trace elements:","Nitrogen source:\n[N]:\n[P]:\npH:\nOther nutrients/trace elements:","Nitrogen source:\n[N]:\n[P]:\npH:\nOther nutrients/trace elements:","mol/L",null,"NA","NA","NA","NA"]
[null,"FOA",null,"% CO2 from DAC",{"$nulls":4},"% of CO2 into cultivation system",null,"Scale: ","Scale: ","Scale: ","Scale: "]
[null,"FOA",null,"Carbon Utilization Efficiency",{"$nulls":4},"g CO2/g AFDW Algae Biomass",null,"Scale: ","Scale: ","Scale: ","Scale: "]
[null,"FOA",null,"Cost of CO2",{"$nulls":4},"$/g of CO2 into cultivation system",null,"Scale: ","Scale: ","Scale: ","Scale: "]
[null,"FOA",null,"Toolkit Delivery",{"$nulls":4},"N/A",null,"Scale: ","Scale: ","Scale: ","Scale: "]

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["NOT DUE AT APPLICATION"]
["APEX Challenge Evaluation Criteria\nThe APEX Challenge performance evaluation criteria outlined below support cultivation- and commercialization-enabling biological innovation. The evaluation criteria were informed by subject matter experts during the BETO sponsored Algal Biology Toolbox Workshop."]
["The applicant does not need to provide this data at the time of application. Note that these criteria may be subject to change after the publication of this FOA. Any updates to these criteria will be distributed to selected projects ahead of reporting."]
["Table 1: Strain & Consortia Attributes"]
["Biological Stressors"]
["Was culture exposed to pests?",null,"Species composition and effect on culture performance:"]
[]
["Was culture exposed to predators?",null,"Species composition and effect on culture performance:"]
[]
["Water Chemistry"]
["What was the salinity of the cultivation water?",null,"Effect on culture performance and sustainability:"]
[]
["What water source was used for cultivation?",null,"Effect on culture performance and sustainability:"]
[]
["What was the pH of the cultivation water?",null,"Effect on culture performance and sustainability:"]
[]
["Abiotic Stressors"]
["Was culture exposed to extremes in water temperature or temperature fluctuation?",null,"Effect on culture performance:"]
[]
["Was culture exposed to extremes in light intensity or light intensity fluctuation?",null,"Effect on culture performance:"]
[]
["Was culture exposed to precipitation?",null,"Effect on culture performance:"]
[]
["Was culture exposed to oxygen tolerance stress?",null,"Effect on culture performance:"]
[]
[]
["Table 2: Cultivation Attributes"]
["Nutrient Source"]
["What media was used?",null,"Would this recipe be used in commercialization? (include cost as a consideration)"]
[]
["What was the inorganic carbon source?",null,"Effect on culture performance and sustainability:"]
[]
["Was organic carbon used? If so, what was the source?",null,"Effect on culture performance and sustainability:"]
[]
["What was the nitrogen source?",null,"Effect on culture performance and sustainability:"]
[]
["What was the phosphorous source?",null,"Effect on culture performance and sustainability:"]
[]
["What additional macronutrients, micronutrients, or trace elements were used?",null,"Effect on culture performance and sustainability:"]
[]
["Cultivation Process Management"]
["Describe the cultivation system used.","What was the modification?","Would this system be used in commercialization?"]
[]
["What was the culture inoculation density?","What was the modification?","Effect on culture performance and sustainability:"]
[]
["Was a crop protection application used? If so, what was the strategy?","What was the modification?","Effect on culture performance and sustainability:"]
[]
["How frequently were cultures harvested?","What was the modification?","Effect on culture performance and sustainability:"]
[]
[]
["Table 3: APEX Project Performance"]
["Biological Performance"]
["Describe the genetic and/or ecological stability of the strain/consortium of interest, if measured.",null,"Factors contributing to stability:"]
[]
["What was the number and frequency of culture crashes per cultivation trial?",null,"Expected cause of crash:*"]
[]
["Process Performance"]
["What was the mean time between failure of cultures?*",null,"Description of culture restart strategy:"]
[]
["Describe the ease of harvest (settling rate or equivalent metric).",null,"Would this system be used in commercialization?"]
[]
["Yield Performance"]
["What was the growth rate?",null,"Comparison of interim and final validation results. Include description of common or deviating independent variables between validations:"]
[]
["What was the total biomass yield?",null,"Comparison of interim and final validation results. Include description of common or deviating independent variables between validations:"]
[]
["What was the biomass composition at harvest?",null,"Description of biomass composition, how was composition determined, consistency of composition between final validation runs and to interim validation:"]
[]
["*Indicate human error if it was a contributing factor"]
["Questions about this FOA? Email PEAKFOA@ee.doe.gov.\nProblems with EERE Exchange? Email EERE- EERE-ExchangeSupport@hq.doe.gov Include FOA name and number in\nsubject line."]