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["Proprietary and Business Sensitive Document"]
["DE-FOA-0002203: FY20 Bioenergy Technologies Multi-Topic FOA\nSubtopic 2c: Synergistic Wastewater Integration with Microalgae (SWIM)\n"]
["Instructions: Please Read","Must correspond to App. Work Plan and SOPO","Must correspond to App. Work Plan and SOPO","What will be used to measure success of the proposed task.  ","List the starting KPP measurement that has been achieved or demonstrated to-date - this is the applicant's baseline value.","List an intermediate target to assess progress at the BP2 G/NG decision.","List the final objective target. This should be the goal of the proposed task and should align achieving with the application objective.","Identify the units of measurement. If dimensionless, state N/A. ","Identify the KPP Baseline Measurement source - citation, in-house experiments, etc. For unit operation tests/experiments include hours of steady-state operation and important operating conditions.","List the duration and scale of the Baseline, Intermediate, and Target KPP (e.g., Bench/Lab/Flasks, ePBR, pond volume in liters, pilot or integrated pilot)"]
[null,"Task Number","Title of Proposed Task","Key Performance Parameter (KPP)","Baseline Measurement","Intermediate Target ","Proposal End Target","Units ","Data Basis / Measurement Description","Baseline","Intermediate","Final"]
["Examples ONLY. DELETE IN SUBMITTED TABLE",1,"Improve productivity of Fantastic strain","Biomass productivity",9,12,20,"Ash-free dry weight of algae produced per square meter per day (g/m2/d)","(1) Biomass productivity test protocols will be requested during the  validation process and should include details on climate parameters (i.e., temperature and light) used in growth studies.\n\n(2) Test protocols will be requested and should include the \"data basis / measurement description\" for biomass productivity values. \n\n(3) Outdoor Productivities should be calculated using the 2016 SOT  \"Experimental duration – grow out – final harvest\" calculation protocol outlined in the 2016 SOT report  (Knoshaug, et al., Use of Cultivation Data ... State of Technology Assessments, NREL, October 2016).","Duration: 28 days\n\nScale:  500 mL ePBRs ","Duration: 28 days\n\nScale:  4.2 m2  outdoor ponds","Duration: 28 days\n\nScale:  4.2 m2  outdoor ponds"]
[null,1,"Improve productivity of Fantastic strain","Biomass composition ","Algae Strain: Strain A\nAsh (wt.%): 20\nProtein (wt.%): 42\nFAME lipid (wt.%): 15\nTotal Carbohydrates (wt.%): 18\n","Algae Strain: Strain A\nAsh (wt.%): 0\nProtein (wt.%): 42\nFAME lipid (wt.%): 35\nTotal Carbohydrates (wt.%): 18\n","Algae Strain: Strain A\nAsh (wt.%): 0\nProtein (wt.%): 42\nFAME lipid (wt.%): 35\nTotal Carbohydrates (wt.%): 18\n","wt.%","The biomass composition \"data basis / measurement description\" should be the same as the productivity data.","Duration: 500 mL ePBRs\n\nScale:  4.2 m2  outdoor ponds","Duration: 28 days\n\nScale:  4.2 m2  outdoor ponds","Duration: 28 days\n\nScale:  4.2 m2  outdoor ponds"]
[null,1,"Improve productivity of Fantastic strain","SUPER gene and protein expression","No SUPER gene expression","mRNA expression of SUPER gene","Expressed SUPER protein and expected phenotype","N/A","PCR","lab-scale/flask","lab-scale/flask","lab-scale/flask and 500 mL ePBRs"]
[null,2,"Improving Amazing strain through designer microbial ecology","Biomass productivity","\"Axenic\" culture: baseline productivity = 9","Microbial consortium with algae: productivity = 12","Microbial consortium with algae: productivity = 20","Ash-free dry weight of algae produced per square meter per day (g/m2/d)","(1) Biomass productivity test protocols will be requested during the  validation process and should include details on climate parameters (i.e., temperature and light) used in growth studies.\n\n(2) Test protocols will be requested and should include the \"data basis / measurement description\" for biomass productivity values. \n\n(3) Outdoor Productivities should be calculated using the 2016 SOT  \"Experimental duration – grow out – final harvest\" calculation protocol outlined in the 2016 SOT report  (Knoshaug, et al., Use of Cultivation Data ... State of Technology Assessments, NREL, October 2016).","Duration: 28 days\n\nScale:  500 mL ePBRs ","Duration: 28 days\n\nScale:  4.2 m2  outdoor ponds","Duration: 28 days\n\nScale:  4.2 m2  outdoor ponds"]
[null,2,"Improving Amazing strain through designer microbial ecology","Biomass composition ","Algae Strain: Amazing grown alone\nAsh (wt.%): 20\nProtein (wt.%): 42\nFAME lipid (wt.%): 15\nTotal Carbohydrates (wt.%): 18\n","Algae Strain: Amazing grown with consortium\nAsh (wt.%): 20\nProtein (wt.%): 42\nFAME lipid (wt.%): 15\nTotal Carbohydrates (wt.%): 18\n","Algae Strain: Amazing grown with consortium\nAsh (wt.%): 20\nProtein (wt.%): 42\nFAME lipid (wt.%): 15\nTotal Carbohydrates (wt.%): 18\n","wt.%","The biomass composition \"data basis / measurement description\" should be the same as the productivity data.","Duration: 28 days\n\nScale:  500 mL ePBRs ","Duration: 28 days\n\nScale:  4.2 m2  outdoor ponds","Duration: 28 days\n\nScale:  4.2 m2  outdoor ponds"]
[null,3,"Gene annotation toolkit development","Accurate biological pathway mapping","No pathway maps for Fantastic strain"," Identification of the potential genes/proteins involved in biological pathway affected by SUPER gene expression","Full biological pathway map of pathways affected by SUPER gene expression","N/A","Mapping visualization; sequence alignments","Mapping software; computational analysis","Mapping software; computational analysis","Mapping software; computational analysis"]
["Please fill in the Required FOA Specific Key Performance Metrics listed here","FOA",null,"Biomass productivity",null,null,null,"Ash-free dry weight of algae produced per square meter per day (g/m2/d)",null,"Duration: \n\nScale: ","Duration: \n\nScale:  ","Duration: \n\nScale:  "]
[null,"FOA",null,"Biomass composition ","Algae Strain:\nAsh (wt.%):\nProtein (wt.%):\nLipid (wt.%):\nTotal Carbohydrates (wt.%):\n","Algae Strain:\nAsh (wt.%):\nProtein (wt.%):\nLipid (wt.%):\nTotal Carbohydrates (wt.%):\n","Algae Strain:\nAsh (wt.%):\nProtein (wt.%):\nLipid (wt.%):\nTotal Carbohydrates (wt.%):\n","wt.%","The biomass composition \"data basis / measurement description\" should be the same as the productivity data.","Duration: \n\nScale: ","Duration: \n\nScale:  ","Duration: \n\nScale:  "]
[null,"FOA",null,"Calculated fuel yield based on achieved productivity and biomass composition",null,null,null,"gasoline-gallon equivalency (GGE)",null,"NA","NA","NA"]
[null,"FOA",null,"Energy requirement per amount of pollutant removed",null,null,null,"kWh/kg pollutant removed",null,"Duration: \n\nScale: ","Duration: \n\nScale: ","Duration: \n\nScale: "]
[null,"FOA",null,"Nutrient Recovery Improvement measured by cost per ton of recovered N and/or P",null,null,"At least 20% of Baseline Measurement.","Cost per ton of recovered N and/or P ($/ton)",null,"Duration: \n\nScale: ","Duration: \n\nScale: ","Duration: \n\nScale: "]
[null,"FOA",null,"Yield of algal feedstock ",null,null,"At least 20% of Baseline Measurement.","ton of algae/million gallons-per day (MGD) of treated water",null,"Duration: \n\nScale: ","Duration: \n\nScale: ","Duration: \n\nScale: "]
[null,"FOA",null,"Effluent total phosphorus concentration after remediation",null,null,"At least 0.3 mg/L","mg/L",null,"Duration: \n\nScale: ","Duration: \n\nScale: ","Duration: \n\nScale: "]
["Add rows and Insert additional project specific KPPs below, if required"]